Human sTNFsR II PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human soluble TNFsR? in cell culture supernates, serum, plasma( heparin, EDTA, citrate) and urine.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human sTNFsR?
Protein name: Tumor necrosis factor receptor superfamily member 1B
Gene Full Name: tumor necrosis factor receptor superfamily, member 1B
abbreviation: TNFRSF1B
Background: Tumor necrosis factor receptor p75(TNF-R p75, or TNF-R2) is a 75-kDa type I transmembrane protein expressed predominantly on cells of hematopoietic lineage. TNF-R p75 belongs to the TNF receptor superfamily characterized by cysteine-rich extracellular regions composed of three to six disulfide-linked domains. The tumor necrosis factor receptor II(TNFRII) gene localizes to 1p36. 2, a genomic region characteristically deleted in neuroblastomas and other malignancies. In addition, TNFRII is the principal mediator of the effects of TNF on cellular immunity, and it may cooperate with TNFRI in the killing of nonlymphoid cells. The standard product used in this kit is recombinant human sTNFRII, consisting of 24-206 amino acid sequence with the molecular mass of 20KDa.
ID:P20333
ID:7133

alias

CD120b|Etanercept|p75|p80 TNF alpha receptor|TBP2|TBPII|TNF R2|TNFBR|TNFR-II|TNFR2|TNFRII|Tumor necrosis factor binding protein 2|Tumor necrosis factor receptor 2|Tumor necrosis factor receptor superfamily member 1B|Tumor necrosis factor receptor superfamily member 1b, membrane form|Tumor necrosis factor receptor type II

Origin

Nativ and recombinant human sTNFsR?

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, L23-D257

sensitivity

<2pg/ml

detection range

7.8pg/ml-500pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|63|125|257|78|134|269
Standard deviation|3.09|7.13|9.25|4.76|9.65|14
CV(%)|4.9|5.7|3.6|6.1|7.2|5.2

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human sTNFsR? ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sTNFsR? has been precoated onto 96-well plates. Standards(NSO, L23-D257) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sTNFsR? is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sTNFsR? amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml human sTNFsR? standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA, citrate) or urine to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human sTNFsR? standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|7.8|15.6|31.3|62.5|125|250|500 O.D.|0.024|0.076|0.127|0.200|0.384|0.777|1.422|2.495

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human sTNFR II antibody|1
ST0000-10|Lyophilized recombinant human sTNFR II standard|10ng/tubex2
AR1107|Biotinylated anti- human sTNFR II antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4