Human IL1R2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL1R2 in cell culture supernates and serum.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL1R2
Protein name: Interleukin-1 receptor type 2
Gene Full Name: interleukin 1 receptor, type II
abbreviation: IL1R2
Background: Interleukin 1 receptor, type II(IL1R2) also known as CD121b(Cluster of Differentiation 121b) is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. It is mapped to 2q11.2. IL1R2 binds interleukin alpha(IL1A), interleukin beta(IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. The secreted IL1R2 recruits secreted IL1RAP with high affinity, and this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.
ID:P27930
ID:7850

alias

IL1 Receptor II|Antigen CDw121b|CD121|CD121 antigen-like family member B|CD121b|CD121B Antigen|CDw121b|IL-1R-2|IL-1R-beta|IL-1R2|IL-1RT-2|IL-1RT2|IL1 R2|IL1 receptor beta chain|IL1R beta|Il1r-2|IL1R2|IL1R2_HUMAN|IL1RB|Interleukin 1 receptor type II precursor|Interleukin 1 receptor, beta, type II|Interleukin 1 receptor, type II|Interleukin-1 receptor beta|Interleukin-1 receptor type 2|Interleukin-1 receptor type II|MGC137383|MGC47725|OTTHUMP00000207969|p68|Type II interleukin 1 receptor beta|Type II interleukin-1 receptor, beta

Origin

Nativ and recombinant human IL1R2

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

sf21, F14-E343

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|85|392|750|96|414|763
Standard deviation|3.91|12.2|28.5|5.95|22.77|38.9
CV(%)|4.6|3.1|3.8|6.2|5.5|5.1

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL1R2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R2 has been precoated onto 96-well plates. Standards(sf21, F14-E343) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R2 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL1R2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL1R2 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.066|0.088|0.144|0.254|0.544|1.007|1.821|2.388

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL1R2 antibody|1
ST0000-10|Lyophilized recombinant human IL1R2 standard|10ng/tubex2
AR1107|Biotinylated anti- human IL1R2 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4