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Boster
For quantitative detection of human IL-4 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-4Protein name: Interleukin-4Gene Full Name: interleukin 4abbreviation: IL4Background: Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa. ID:P05112 ID:3565
B cell growth factor 1|B cell stimulatory factor 1|BCGF 1|BCGF1|Binetrakin|BSF 1|BSF1|IL 4|Interleukin 4|Lymphocyte stimulatory factor 1|MGC79402|Pitrakinra
Nativ and recombinant human IL-4
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, H25-S153
<1.5pg/ml
15.6pg/ml-1000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|55.4|361|638|45.8|375|656 Standard deviation|2.5|14.8|37.64|2.977|24|46.58 CV(%)|4.5|4.1|5.9|6.5|6.4|7.1
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-4 has been precoated onto 96-well plates. Standards(E.coli, H25-S153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-4 amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human IL-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-4 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.018|0.039|0.073|0.151|0.377|0.908|1.872|2.627
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-4 antibody|1ST0000-10|Lyophilized recombinant human IL-4 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-4 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <1.5pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-4 (IL4) |
Detection Range | 15.6pg/ml-1000pg/ml |
Alias | B cell growth factor 1, B cell stimulatory factor 1, BCGF 1, BCGF1, Binetrakin, BSF 1, BSF1, IL 4, Interleukin 4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra |