Human IL-33 Quantitation Kit

Brand

GENWAY

Overview

Sample Type: Serum or plasma. For the quantitative determination of Human IL-33 levels in serum or other biological samples. Connective Tissue Cells Immune System Autoimmune Diseases Blood Cells Cultured . Product Name: Human IL-33. Sample Type: Serum or plasma. # of Samples: 2X96 Assays. Introduction: Interleukin-1 (IL-1) family members such as IL-19 and IL-18 play important roles in host defense immune regulation neuronal injury and inflammation (1 2). Recently a new member of this family IL-33 also named Nuclear Factor from High Endothelial Venules (NF-HEV) (3) has been reported. It mediates its biological effects via IL-1 receptor ST2 (4) an orphan receptor of the IL-1 receptor family (5) and activates NF-kB and MAPK pathways. IL-33 is synthesized in response to cytokines such as IL-1: and plays an important role in TH2-associated immunology. IL-33 expression drives production of TH2-associated cytokines from in vitro polarized TH2 cells and induces the expression of IL-4 IL-5 and IL-13 in vivo. Overexpression of IL-33 in mice leads to severe pathological changes in mucosal organs. Structurally IL-33 is closest to IL-18 rather than IL-1. The human IL-33 is expressed with a prodomain (aa 1-111) that is cleaved by caspase-1 to produce a 18kDa protein (112-270) (6). Storage and Stability: -20 degree C. Product Name: Human IL-33. Range of Detection: 500 - 0. 7 ng/ml. Shelf life: One year from date of receipt. Assay Condition: The kit performance has been optimized for the stated protocol using the materialslisted and standard dilutions from 500-0. 7 ng/ml of human IL-33. For alternativeassay conditions the operator must determine appropriate dilutions of reagents. ELISA assay reactivity is sensitive to any variation in operator pipetting andwashing techniques incubation time and temperature composition of reagents andkit age. Adjustments may be required to position the standard curve and/or samplesin the desired detection range. Country of Origin: United States of AmericaAssay Use: For Research Use Only (RUO). Not for diagnostic or therapeutic use in humans or animals. Not for animal or human consumption. Function: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88 IRAK1 IRAK4 and TRAF6 followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2 MAPK14 and MAPK8. Induces T helper type 2-associated cytokines. Subcellular Location: Secreted. Tissue Specificity: Expressed at high level in high endothelial venules found in tonsils Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. Ptm: Proteolytically converted to a mature form by CASP1. Similarity: Belongs to the IL-1 family. Highly divergent. Product Protocol: To see this product used in a peer reviewed journal click the following link:Serum levels of IL-33 and soluble ST2 and their association with disease activity in systemic lupus erythematosus

spezie

Human

detection target

IL-33

pack size

2x96 Wells