Human IL-1RA PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-1RA in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IL-1RA
Protein name: Interleukin-1 receptor antagonist protein
Gene Full Name: interleukin 1 receptor antagonist
abbreviation: IL1RN
Background: The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.
ID:P18510
ID:3557

alias

Anakinra|DIRA|ICIL 1RA|ICIL-1RA|IL 1ra|IL 1RN|IL-1ra|IL-1RN|IL1 inhibitor|IL1F3|IL1RA|IL1RA_HUMAN|IL1RN|Interleukin 1 receptor antagonist|Interleukin 1 receptor antagonist protein|Interleukin-1 receptor antagonist protein|Intracellular IL 1 receptor antagonist type II|Intracellular interleukin 1 receptor antagonist|IRAP|MGC10430|MVCD4|Type II interleukin 1 receptor antagonist

Origin

Nativ and recombinant human IL-1RA

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, R26-E177

sensitivity

<2pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|80|574|1395|76|509|1213
Standard deviation|4.64|25.83|61.38|7|37.16|77.6
CV(%)|5.8|4.5|4.4|9.2|7.3|6.4

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-1RA ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1RA has been precoated onto 96-well plates. Standards(E.coli, R26-E177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1RA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1RA amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL-1RA standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL-1RA standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.007|0.128|0.224|0.456|0.842|1.354|1.973|2.272

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-1RA antibody|1
ST0000-10|Lyophilized recombinant human IL-1RA standard|10ng/tube�2
AR1107|Biotinylated anti- human IL-1RA antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4