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Boster
For quantitative detection of human IL-18R1 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
human IL-18R1Protein name: Interleukin-18 receptor 1Gene Full Name: interleukin 18 receptor 1abbreviation: IL18R1Background: The interleukin-18 receptor 1(IL-18R1), also known as CDw218a(cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2(IL-1Rrp2), and IL1RL1(T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18(IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. ID:Q13478 ID:8809
CD218 antigen-like family member A|CD218a|CDw218a|CDw218a antigen|IL 1Rrp|IL-18R-1|IL-18R1|IL-1Rrp|IL1 receptor related protein|IL1 receptor-related protein|IL18R_HUMAN|IL18R1|IL18RA|Il18ralpha|IL1R-rp|IL1RRP|Interleukin 18 receptor 1|Interleukin 18 receptor, alpha chain|Interleukin-18 receptor 1
Nativ and recombinant human IL-18R1
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, C22-R329
<10pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|250|625|1255|253|612|1078 Standard deviation|13.2|38.7|70.2|20.7|45.9|76.5 CV(%)|5.3|6.2|5.6|8.2|7.5|7.1
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-18R1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18R1 has been precoated onto 96-well plates. Standards(NSO, C22-R329) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18R1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18R1 amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human IL-18R1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-18R1 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.016|0.076|0.139|0.258|0.502|0.891|1.558|2.209
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-18R1 antibody|1ST0000-10|Lyophilized recombinant human IL-18R1 standard|10ng/tube�2AR1107|Biotinylated anti- human IL-18R1 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-18 receptor 1 (IL18R1) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | CD218 antigen-like family member A, CD218a, CDw218a, CDw218a antigen, IL 1Rrp, IL-18R-1, IL-18R1, IL-1Rrp, IL1 receptor related protein, IL1 receptor-related protein, IL18R_HUMAN, IL18R1, IL18RA, Il18ralpha, IL1R-rp, IL1RRP, Interleukin 18 receptor 1, Int |