Human IL-1 alpha ELISA Kit

Brand

Cell Sciences

Description

Monocytes are the main source of secreted Interleukin-1 (IL1). They express predominantly IL1-beta (IL1B) while Human keratinocytes express large amounts of IL1-alpha (IL1A). IL1 is produced also by activated macrophages from different sources (alveolar macrophages, Kupffer cells, adherent spleen and peritoneal macrophages) and also by peripheral neutrophil granulocytes. IL1-alpha and IL1-beta are biologically more or less equivalent pleiotropic factors that act locally and also systemically. The main biological activity of IL1 is the stimulation of T-helper cells, which are induced to secrete IL2 and to express IL2 Receptors. The Human IL1-alpha ELISA Kit is an, in vitro, enzyme-linked immunosorbent assay for the quantitative measurement of Human IL1-alpha in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Human IL1-alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1-alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and Biotinylated Anti-Human IL1-alpha antibody is added. After washing away unbound Biotinylated antibody, HRP-Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1-alpha bound.

Storage and Stability

May be stored for up to 6 months at 2-8C from the date of shipment. Standard should be stored below -20C (-80C recommended) after reconstitution. Opened Microplate Wells and reagents may be store for up to 1 month at 2-8C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge of zipseal.

Sensitivity

The minimum detectable dose of IL1A is typically less than 2 pg/ml.

spezie

Human

Cross Reaction

Human

detection target

Human IL-1 alpha
Antigen: IL1A/IL-1 alpha

alias

hematopoietin-1, IL1-alpha, interleukin-1-alpha, IL1F1, IL-1 alpha,

sensitivity

The minimum detectable dose of IL1A is typically less than 2 pg/ml.

sample size

1

pack size

PP

UNSPSC

41116126 and E