Human IFN gamma PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IFN gamma in cell culture supernates and serum.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human IFN gamma
Protein name: Interferon gamma
Gene Full Name: interferon, gamma
abbreviation: IFNG
Background: Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
ID:P01579
ID:3458

alias

IFG|IFI|IFN gamma|IFN Immune|IFNG|Interferon gamma|Interferon Gamma Precursor|Macrophage Activating Factor|MAF|T Cell Interferon|Type II Interferon

Origin

Nativ and recombinant human IFN gamma

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, Q24-Q166

sensitivity

<2pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|98.5|264|579|107|315|589
Standard deviation|5.32|9.24|22.00|8.667|20.79|31.81
CV(%)|5.4|3.5|3.8|8.1|6.6|5.4

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IFN gamma ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IFN gamma has been precoated onto 96-well plates. Standards(E.coli, Q24-Q166) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IFN gamma is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IFN gamma amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human IFN gamma standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IFN gamma standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.011|0.056|0.098|0.183|0.366|0.646|1.382|2.405

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IFN gamma antibody|1
ST0000-10|Lyophilized recombinant human IFN gamma standard|10ng/tubex2
AR1107|Biotinylated anti- human IFN gamma antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4