Human GM-CSF/CSF2 ELISPOT Kit w/o plates

Brand

Cell Sciences

Description

The cytokine ELISPOT (Enzyme-Linked ImmunoSPOT) assay is designed to enumerate cytokine-secreting cells insingle cell suspensions of lymphoid tissue, central nerve system (CNS) tissue, bone marrow or preparations ofperipheral blood mononuclear cells (PBMCs). The assay has the advantage of detecting only activated/memory Tcellsand has the ability to detect cytokine release in response to antigen by a single cell thereby permitting directcalculation of responder T-cell frequencies. The high sensitivity and easy performance, allowing the determination ofpeptide-reactive T-cells without prior in vitro expansion, makes the ELISPOT assay eminently well suited to monitor Tcellresponses. The higher sensitivity of ELISPOT in comparison to that of ELISA or intracellular staining is due to theplate-bound antibodies directly capturing the cytokine released by the cell before it is diluted in the supernatant,trapped by high-affinity receptors or degraded by proteases. The sensitivity of the assay lends itself to measurementof very low frequencies of cytokine-secreting cells (1/300,000).

Kit Content

Coating antibody, Biotinylated detection antibody, Streptavidin-HRP, AEC coloring system, Blocking stock solution R, Dilution buffer R, Tween-20

spezie

Human

Cross Reaction

Human

detection target

Human GM-CSF/CSF2
Antigen: CSF2/GM-CSF

alias

GMCSF, mgC131935, mgC138897, granulocyte-macrophage colony-stimulating factor, CSF, molgramostin, sargramostim, colony-stimulating factor, granulocyte-macrophage colony stimulating factor

sample size

5

pack size

PP

UNSPSC

41116126 and ES