Profile Informations
Login Datas
or login
Boster
For quantitative detection of human G-CSF in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human G-CSFProtein name: Granulocyte colony-stimulating factorGene Full Name: colony stimulating factor 3 (granulocyte)abbreviation: CSF3Background: Granulocyte colony-stimulating factor(G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa. ID:P09919 ID:1440
C17orf33|Colony stimulating factor 3 (granulocyte)|CSF 3|CSF beta|CSF3|CSF3OS|Csfg|Filgrastim|GCSA|GCSF|Granulocyte colony stimulating fact|Lenograstim|Macrophage granulocyte inducer 2|MGC45931|MGI 2|MGI IG|OTTMUSP00000006785|Pluripoietin|RP23-395E10.4
Nativ and recombinant human G-CSF
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, T31-P204
<4pg/ml
31.2pg/ml-2000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|145|727|1436|153|718|1537 Standard deviation|5.51|30.53|58.88|8.42|46.67|104.5 CV(%)|3.8|4.2|4.1|5.5|6.5|6.8
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human G-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for G-CSF has been precoated onto 96-well plates. Standards(E.coli, T31-P204) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for G-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human G-CSF amount of sample captured in plate.
Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human G-CSF standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human G-CSF standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.011|0.135|0.279|0.464|0.928|1.496|1.956|2.308
Catalog number|Description|Quantity-|96-well plate precoated with anti- human G-CSF antibody|1ST0000-10|Lyophilized recombinant human G-CSF standard|10ng/tubex2AR1107|Biotinylated anti- human G-CSF antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
---|---|
Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <4pg/ml |
Specific Against | Human |
Antigen Name | Granulocyte colony-stimulating factor (CSF3) |
Detection Range | 31.2pg/ml-2000pg/ml |
Alias | C17orf33, Colony stimulating factor 3 (granulocyte), CSF 3, CSF beta, CSF3, CSF3OS, Csfg, Filgrastim, GCSA, GCSF, Granulocyte colony stimulating fact, Lenograstim, Macrophage granulocyte inducer 2, MGC45931, MGI 2, MGI IG, OTTMUSP00000006785, Pluripoietin |