Human G-CSF/CSF3 ELISA Kit

Brand

Cell Sciences

Description

G-CSF (Granulocyte colony stimulating factor), which is an Oglycosylated 19.6 kDa glycoprotein, is secreted by monocytes,macrophages and neutrophils after cell activation. G-CSF is produced also by stromal cells, fibroblasts, and endothelial cells. Itstimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophil/granulocyte lineage in adose-dependent manner. A combination of IL-4 with G-CSF has been shown to lead to synergistic suppression of thegrowth of some human leukemic cell lines. The main and most important clinical application of G-CSF is probably the treatment oftransient phases of leukopenia following chemotherapy and/or radiotherapy.The Cell Sciences Human G-CSF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbentassay for the quantitative measurement of human G-CSF in serum, plasma, cell culture supernatants and urine. This assayemploys an antibody specific for human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells andG-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antihumanG-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells.The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GCSFbound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

spezie

Human

Cross Reaction

Human

detection target

Human G-CSF/CSF3
Antigen: CSF3/G-CSF

alias

C17orf33, CSF3OS, GCSF, mgC45931, granulocyte colony-stimulating factor, filgrastim, lenograstim, pluripoietin

sample size

1

pack size

PP

UNSPSC

41116126 and E