Human DCR3/TNFRSF6B PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human DCR3 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human DCR3
Protein name: Tumor necrosis factor receptor superfamily member 6B
Gene Full Name: tumor necrosis factor receptor superfamily, member 6b, decoy
abbreviation: TNFRSF6B
Background: Decoy receptor 3 (Dcr3), also known as tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), TR6 and M68, is a soluble protein of the tumor necrosis factor receptor superfamily which inhibits Fas ligand-inducedapoptosis. This gene belongs to the tumor necrosis factor receptor superfamily. It acts as a decoy receptor that competes with death receptors for ligand binding. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL1A. Overexpression of this gene has been noted in various tumors e.g. gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes.
ID:O95407
ID:8771

alias

DcR3|Decoy Receptor 3|Decoy receptor for Fas ligand|DJ583P15.1.1|M68|M68E|OTTHUMP00000031583|TNF6B_HUMAN|TNFRSF6B|TR6|Tumor necrosis factor receptor superfamily member 6B|Tumor necrosis factor receptor superfamily member 6b decoy|Tumor necrosis factor receptor superfamily member 6b decoy|UNQ186/PRO212

Origin

Nativ and recombinant human DCR3

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

sf21, V24-H300

sensitivity

<10pg/ml

detection range

31.2pg/ml-2000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|45|177|705|51|193|833
Standard deviation|2.47|8.67|38.775|3.62|13.31|49.14
CV(%)|5.5|4.9|5.5|7.1|6.9|5.9

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human DCR3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DCR3 has been precoated onto 96-well plates. Standards(sf21, V24 - H300) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DCR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DCR3 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml human DCR3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human DCR3 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000 O.D.|0.086|0.176|0.284|0.400|0.696|1.145|1.629|2.207

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human DCR3 antibody|1
ST0000-10|Lyophilized recombinant human DCR3 standard|10ng/tube�2
AR1107|Biotinylated anti- human DCR3 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4