Human CD44 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD44 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human CD44
Protein name: CD44 antigen
Gene Full Name: CD44 molecule(Indian blood group)
abbreviation: CD44
Background: CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
ID:P16070
ID:960

alias

LHR|BA-1|CD 44|CD44|CD44 antigen|CD44 molecule(Indian blood group)|CD44 molecule|CD44_HUMAN|CDw44|Cell surface glycoprotein CD44| chondroitin sulfate proteoglycan 8|CSPG8|ECMR-III|Epican|Extracellular matrix receptor III|GP90 lymphocyte homing/adhesion receptor| HCELL|hematopoietic cell E- and L-selectin ligand|Heparan sulfate proteoglycan|Hermes antigen |homing function and Indian blood group system|HSA|HUTCH-I|HUTCH1|Hyaluronate receptor|IN|LHR|MC56|MDU2|MDU3|MGC10468|MIC4|MUTCH1|PGP-1|PGP-I|PGP1|Phagocytic glycoprotein 1| Phagocytic glycoprotein I

Origin

Nativ and recombinant human CD44

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO,Gln21-Pro220

sensitivity

<20pg/ml

detection range

0.78ng/ml-50ng/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|7.25|15|38|8.3|19.8|45
Standard deviation|0.33|0.66|1.94|0.62|1.37|3.33
CV(%)|4.6|4.4|5.1|7.5|6.9|7.4

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD44 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD44 has been precoated onto 96-well plates. Standards(NSO, Gln21 - Pro220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD44 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD44 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml human CD44 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human CD44 standard solution and each sample is measured in duplicate.

tipical result

Concentration(ng/ml)|0|0.78|1.56|3.12|6.25|12.5|25|50 O.D.|0.051|0.101|0.134|0.243|0.439|0.829|1.482|2.472

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD44 antibody|1
ST0000-50|Lyophilized recombinant human CD44 standard|50ng/tube�2
AR1107|Biotinylated anti- human CD44 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4