Human CD40/TNFRSF5 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human CD40 in cell culture supernates, serum, plasma(heparin, EDTA), saliva, urine and human milk.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human CD40
Protein name: Tumor necrosis factor receptor superfamily member 5
Gene Full Name: CD40 molecule, TNF receptor superfamily member 5
abbreviation: CD40
Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells, most mature B-cell malignancies, and some early B-cell acute lymphocytic leukemias, but is not expressed on plasma cells. The CD40 gene is localized to the long arm of chromosome 20, bands q12-q13.2. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation.
ID:P25942
ID:958

alias

AI326936|B cell associated molecule|B cell associated molecule CD40|B cell surface antigen CD40|Bp50|CD 40|CD40 antigen|CD40 antigen (TNF receptor superfamily member 5)|CD40 molecule|CD40 molecule TNF receptor superfamily member 5|CD40 protein|CD40 type II isoform|CD40L receptor|CDw40|GP39|HIGM1|IGM|MGC9013|Nerve growth factor receptor related B lymphocyte activation molecule|NFRSF5|OTTHUMP00000031699|OTTHUMP00000031700|p50|T BAM|TBAM|TNF receptor superfamily member 5|TNFRSF5|TRAP|Tumor necrosis factor receptor superfamily member 5|Tumor necrosis factor receptor superfamily member 5 precursor|Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a

Origin

Nativ and recombinant human CD40

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, E21-R193

sensitivity

<1pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|93|325|538|101|357|529
Standard deviation|4.8|19.2|28.5|6.9|26.4|32.8
CV(%)|5.2|5.9|5.3|6.8|7.4|6.2

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human CD40 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD40 has been precoated onto 96-well plates. Standards(NSO, E21-R193) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD40 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD40 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human CD40 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA), saliva, urine or human milk to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human CD40 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.010|0.077|0.147|0.221|0.419|0.997|1.620|2.394

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human CD40 antibody|1
ST0000-10|Lyophilized recombinant human CD40 standard|10ng/tube�2
AR1107|Biotinylated anti- human CD40 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4