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Boster
For quantitative detection of human CD26 in cell culture supernates, serum, plasma(heparin, EDTA), saliva and urine.
Human
There is no detectable cross-reactivity with other relevant proteins.
Human CD26Protein name: Dipeptidyl peptidase 4Gene Full Name: dipeptidyl-peptidase 4abbreviation: DPP4Background: Dipeptidyl peptidase-4(DPP4), also known as adenosine deaminase complexing protein 2 or CD26(cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others. ID:P27487 ID:1803
ADABP|ADCP-2|ADCP2|Adenosine deaminase complexing protein 2|CD26|Dipeptidyl peptidase 4|Dipeptidyl peptidase 4 soluble form|Dipeptidyl peptidase IV|Dipeptidyl peptidase IV membrane form|Dipeptidyl peptidase IV soluble form|Dipeptidylpeptidase 4|DPP 4|DPP IV|DPP4|DPP4_HUMAN|DPPIV|T-cell activation antigen CD26|TP103
Nativ and recombinant human CD26
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
NSO, D34-P766
<10pg/ml
312pg/ml-20000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(ng/ml)|1.58|7.23|12.07|1.66|7.59|12.83 Standard deviation|0.098|0.513|0.881|0.126|0.645|1.052 CV(%)|6.2|7.1|7.3|7.6|8.5|8.2
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human CD26 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD26 has been precoated onto 96-well plates. Standards(NSO, D34-P766) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD26 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD26 amount of sample captured in plate.
Aliquot 0.1ml per well of the 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human CD26 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma( heparin, EDTA) saliva or urine to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human CD26 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|312|625|1250|2500|5000|10000|20000 O.D.|0.002|0.082|0.166|0.297|0.506|1.007|1.630|2.311
Catalog number|Description|Quantity-|96-well plate precoated with anti- human CD26 antibody|1ST0000-20|Lyophilized recombinant human CD26 standard|20ng/tubex2AR1107|Biotinylated anti- human CD26 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Dipeptidyl peptidase 4 (DPP4) |
Detection Range | 312pg/ml-20000pg/ml |
Alias | ADABP, ADCP-2, ADCP2, Adenosine deaminase complexing protein 2, CD26, Dipeptidyl peptidase 4, Dipeptidyl peptidase 4 soluble form, Dipeptidyl peptidase IV, Dipeptidyl peptidase IV membrane form, Dipeptidyl peptidase IV soluble form, Dipeptidylpeptidase 4, |