Human B7-H3 / CD276 ELISA Kit

Brand

Sino Biological

short description

For the quantitative determination of Human B7-H3 / CD276 concentration in serum and urine.

spezie

Human

natural samples' content determination

The average concentration of Human B7H3 in 10 normal human serum is 2188.44 +/- 692.04 pg/mL ranging from 1365.12 to 3598.60 pg/mL.The average concentration of Human B7H3 in 6 normal human urine is 390.20 +/- 268.93 pg/mL ranging from 83.33 to 678.93 pg/mL.

Cross Reaction

This assay recognizes both recombinant and natural Human B7H3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

detection target

CD276
Antigene: 11188-H08H
capture antibody type: mouse mAbdetection antibody type

sensitivity

16.20 pg/ml

detection range

23.44-1500 pg/ml

certificate

ISO9001-2008

pack size

96 Wells

storage and Stability

Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA

Principle of the Elisa

Solid Phase Sandwich ELISA

Kit Content

1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution