Human Angiotensinogen / SerpinA8 / AGT ELISA Kit

Brand

Sino Biological

short description

For the quantitative determination of Human Angiotensinogen / SerpinA8 / AGT concentration in serum.

spezie

Human

natural samples' content determination

The average concentration of AGT in 10 normal human serum is 28.26 +/- 6.38 µg/mL ranging from 17.29 to 33.12 µg/mL.

Cross Reaction

This assay recognizes both recombinant and natural Human AGT. SerpinA1 and SerpinI2 were prepared at 250 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 0.81% with 250 ng/mL of recombinant human SerpinA5.

detection target

AGT
Antigene: 10994-H08H
capture antibody type: mouse mAb

sensitivity

42.46 pg/ml

detection range

187.5-12000 pg/ml

certificate

ISO9001-2008

pack size

96 Wells

storage and Stability

Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA

Principle of the Elisa

Solid Phase Sandwich ELISA

Kit Content

1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution