Human ADAM8 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human ADAM8 in cell culture supernates and serum.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human ADAM8
Protein name: Disintegrin and metalloproteinase domain-containing protein 8
Gene Full Name: ADAM metallopeptidase domain 8
abbreviation: ADAM8
Background: A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.
ID:P78325
ID:101

alias

A Disintegrin And Metalloproteinase domain 8|A Disintegrin And Metalloproteinase domain 8|ADAM 8|ADAM 8 precursor|ADAM 8 precursor| ADAM metallopeptidase domain 8|Adam8|ADAM8 protein|ADAM8_HUMAN|CD 156|CD156|CD156a|CD156a antigen|CD156a antigen|Cell surface antigen MS2|Cell surface antigen MS2|Disintegrin and metalloproteinase domain-containing protein 8|Human leukocyte differentiation antigen|Human leukocyte differentiation antigen|Macrophage cysteine rich glycoprotein|Macrophage cysteine rich glycoprotein| MGC134985|MS 2|MS2

Origin

Nativ and recombinant human ADAM8

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, M1-P497

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.22|1.47|2.34|0.27|1.56|2.58
Standard deviation|0.013|0.090|0.185|0.018|0.115|0.214
CV(%)|5.7|6.1|7.9|6.8|7.4|8.3

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human ADAM8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM8 has been precoated onto 96-well plates. Standards(NSO, M1-P497) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM8 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human ADAM8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human ADAM8 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.006|0.060|0.129|0.256|0.442|0.829|1.384|1.979

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human ADAM8antibody|1
ST0000-10|Lyophilized recombinant human ADAM8 standard|10ng/tube�2
AR1107|Biotinylated anti- human ADAM8 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4