Human ACE2 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human ACE2 in cell culture supernates, serum and plasma(heparin).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human ACE2
Protein name: Angiotensin-converting enzyme 2
Gene Full Name: angiotensin I converting enzyme 2
abbreviation: ACE2
Background: AngiotensinI-converting enzyme 2(ACE 2) is a protein belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. By sequence similarity to a sequence in GenBank, this gene is mapped to Xp22.2. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63.
ID:Q9BYF1
ID:59272

alias

ACE 2|ACE related carboxypeptidase|ACE-related carboxypeptidase|ACE2|ACE2_HUMAN|ACEH|Angiotensin converting enzyme 2|Angiotensin converting enzyme homolog|Angiotensin converting enzyme like protein|Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2|Angiotensin I converting enzyme 2|Angiotensin-converting enzyme homolog|DKFZP434A014|EC 3.4.17|metalloprotease MPROT 15|Metalloprotease MPROT15|OTTHUMP00000022963|Processed angiotensin-converting enzyme 2

Origin

Nativ and recombinant human ACE2

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, Q18-S740

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|438|1175|2386|508|1293|2417
Standard deviation|19.71|56.4|124.07|34.54|98.27|188.53
CV(%)|4.5|4.8|5.2|6.8|7.6|7.8

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human ACE2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ACE2 has been precoated onto 96-well plates. Standards(NSO, Q18-S740) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ACE2 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human ACE2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human ACE2 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.154|0.208|0.258|0.347|0.538|0.885|1.440|2.205

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human ACE2 antibody|1
ST0000-10|Lyophilized recombinant human ACE2 standard|10ng/tube�2
AR1107|Biotinylated anti- human ACE2 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30 ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4