Human ACE PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human ACE in cell culture supernates, serum, plasma(heparin) and saliva.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human ACE
Protein name: Angiotensin-converting enzyme
Gene Full Name: angiotensin I converting enzyme
abbreviation: ACE
Background: Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
ID:P12821
ID:1636

alias

ACE 1 | ACE T | Angiotensin converting enzyme | Angiotensin Converting Enzyme 1 | Angiotensinconverting enzyme | Angiotensin-converting enzyme | CD143 antigen | DCP 1 | DCP, DCP1 | Dipeptidyl carboxypeptidase 1 | Dipeptidyl carboxypeptidase I | Kininase II | MVCD3 | P12821 | Peptidyl dipeptidase A

Origin

Nativ and recombinant human ACE

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, L30-L1261

sensitivity

<5pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|0.85|2.73|6.87|0.97|3.42|6.33
Standard deviation|0.036|0.112|0.261|0.671|0.246|0.335
CV(%)|4.2|4.1|3.8|6.9|7.2|5.3

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human ACE ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ACE has been precoated onto 96-well plates. Standards(NSO, L30-L1261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ACE is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ACE amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 313pg/ml, 156pg/ml human ACE standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum, plasma(heparin) or saliva to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human ACE standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.051|0.119|0.204|0.336|0.611|0.929|1.447|2.154

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human ACE antibody|1
ST0000-10|Lyophilized recombinant human ACE standard|10ng/tubex2
AR1107|Biotinylated anti- human ACE antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4