Equine Insulin (INS) ELISA Kit

Brand

DlDevelop

short description

for quantitatively determining the concentration in the customer's samples
Intended Use: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of this index in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

host

Equus caballus; Equine Horse

spezie

Equine

Cross Reaction

This assay has high sensitivity and excellent specificity for detection of this index.
No significant cross-reactivity or interference between this index and analogues was observed.

detection target

INS
Gene ID: 100060077 ID:P01310

sensitivity

7.5 ng/mL

detection range

1.56-100 ng/mL

ISO-certificate nr.

CE and ISO (Nr.:17916Q10078ROS)

pack size

48 and 96 Wells

Reproducibility

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions.

storage and Stability

1.For unopened kit: All the reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer (30 x concentrate) and the Stop Solution should be stored at 4°C. upon receipt while the others should be at -20°C.
2.For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Principle of the Elisa

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to this index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled this index and unlabeled this index (Standards or samples) with the pre-coated antibody specific to this index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of this index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of this index in the sample.

Kit Content

Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 2
Standard 2 Standard Diluent 1x20mL
Detection Reagent A 1x70µL Assay Diluent A 1x12mL
Detection Reagent B 1x120µL Assay Diluent B 1x12mL
TMB Substrate 1x9mL Stop Solution 1x6mL
Wash Buffer (30 x concentrate) 1x20mL Instruction manual 1