Equine IgA

Brand

GENWAY

Overview

Intended Use: The total Horse IgA test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgA in horses’ biological samples. PRINCIPLE OF THE ASSAY The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing anti-IgA antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step the enzyme bound to the immunosorbent is assayed by the addition of a chromo­genic substrate 3 3’ 5 5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus the absorbance at 450 nm is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.

spezie

Equine

detection target

Equine IgA

pack size

1x96 Wells