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Cloud-Clone
Enzyme-linked immunosorbent assay for Antigen Detection.Research focus: Endocrinology; Hormone metabolism; ;
Equus caballus; Equine (Horse)
This assay has high sensitivity and excellent specificity for detection of Adrenomedullin (ADM).
No significant cross-reactivity or interference between Adrenomedullin (ADM) and analogues was observed.
Adrenomedullincoating antibody: Mouse
AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide
The minimum detectable dose of this kit is typically less than 4.92 pg/mL
12.35-1000 pg/mL
ISO 9001:2008, ISO 13485:2003
serum, plasma and other biological fluids
48T, 96T, 5x96T, 10x96T
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin (ADM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin (ADM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
2h
All the kits should stored according to the manual. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree upon receipt while the others should be at 4 degree.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Competitive InhibitionTest principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenomedullin (ADM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenomedullin (ADM) and unlabeled Adrenomedullin (ADM) (Standards or samples) with the pre-coated antibody specific to Adrenomedullin (ADM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample.
Pre-coated, ready to use 96-well strip plate: 1 Plate sealer for 96 wells: 4 Standard: 2 Standard Diluent: 1x20mL Detection Reagent A: 1 Assay Diluent A: 1x12mL Detection Reagent B: 1x120µ L Assay Diluent B: 1x12mL Reagent Diluent: 1x300µ L Stop Solution: 1x6mLTMB Substrate: 1x9mL Wash Buffer (30 x concentrate): 1x20mL Instruction manual:1
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
And then add 50µ L prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
7. Add 50µ L Stop Solution. Read at 450 nm immediately.
Brand | Cloud-Clone |
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Country of Manufacture | China |
Sensitivity | The minimum detectable dose of this kit is typically less than 5.11 pg/mL |
Specific Against | Equine |
Antigen Name | Adrenomedullin (ADM) |
Detection Range | 12.35-1000 pg/mL |
Certified | ISO 9001:2008, ISO 13485:2003 |
Alias | AM/ PAMP/ Proadrenomedullin N-2NA terminal peptide/ ProAM N-terminal 2NA peptide |