Dermatan sulfate ELISA for Plasma

Brand

Lifespan Technologies

short description

Dermatan sulfate (DS) (structure shown) is in the polysaccharide family glycosaminoglycan. DS is found mostly in the skin but also in blood vessels, tendons, heart valves, and pulmonary connective tissue. Heparan sulfate (HS) is a glycosaminoglycan that is found on the surface of most cells as part of proteoglycans. Heparan sulfate is also present in the extracellular matrix. The polysaccharide mediates the interactions between a number of different proteins. The DS / HS ELISA, product number K-6000, is a quantitative enzyme-linked assay designed for the in vitro measurement of DS and/or HS levels in plasma. This assay measures DS / HS directly using a DS / HS binding protein which has been conjugated to HRP. The DS / HS -ELISA is a competitive assay in which the colorimetric signal is inversely proportional to the amount of DS / HS present in the sample. Samples to be assayed are first mixed with the detector-enzyme conjugate in wells of the coated plate. DS / HS in the sample competes with DS / HS bound to the plate for binding of the detector-enzyme conjugate. The concentration of DS / HS in the sample is determined using a standard curve of known amounts of DS / HS.
Normal Plasma Pipettes (8-channel multipipettor recommended) Absorbance microplate reader Dextran and/or Heparan sulfate standards Plate Cover

spezie

Human

detection target

Dermatan sulfate

pack size

1x96 Wells

storage and Stability

Kit can be stored unopened at 4°C for up to six months. Opened and reconstituted solutions can be used for up to one week when stored at 4°C. All components and solutions should be protected from light. Reconstituted detector enzyme conjugate should be used immediately or aliquotted and stored at -80°C.

Kit Content

Coated 96-well plate Detector -Enzyme Conjugate vial Conjugate Diluent TMB Solution Stop Solution, 0.5M H2SO4 Wash Concentrate 10X, (dilute 1 part plus 9 parts water to make TBS plus 0.05% TWEEN 20)