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Cloud-Clone
Chemiluminescent immunoassay for Antigen Detection.Research focus: Infection immunity; Immune molecule; Hematology; ;
Capra hircus; Caprine (Goat)
This assay has high sensitivity and excellent specificity for detection of Immunoglobulin G (IgG).
No significant cross-reactivity or interference between Immunoglobulin G (IgG) and analogues was observed.
Immunoglobulin Gcoating antibody: Mouse
The minimum detectable dose of this kit is typically less than 0.78 ng/mL
1.95-500 ng/mL
ISO 9001:2008, ISO 13485:2003
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
48T, 96T, 5x96T, 10x96T
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Immunoglobulin G (IgG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
2h
All the kits should stored according to the manual. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree upon receipt while the others should be at 4 degree.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Competitive InhibitionTest principle: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Immunoglobulin G (IgG). A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G (IgG) and unlabeled Immunoglobulin G (IgG) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G (IgG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G (IgG) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Immunoglobulin G (IgG) level in the sample or standard.
Pre-coated, ready to use 96-well strip plate: 1 Plate sealer for 96 wells: 4 Standard: 2 Standard Diluent: 1x20mL Detection Reagent A: 1 Assay Diluent A: 1x12mL Detection Reagent B: 1x120µ L Assay Diluent B: 1x12mL Reagent Diluent: 1x300µ L Stop Solution: 1x6mLTMB Substrate: 1x9mL Wash Buffer (30 x concentrate): 1x20mL Instruction manual:1
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
And then add 50µ L prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
7. Read RLU value immediately.
Brand | Cloud-Clone |
---|---|
Country of Manufacture | China |
Sensitivity | The minimum detectable dose of this kit is typically less than 0.78 ng/mL |
Specific Against | Goat |
Antigen Name | CLIA Kit for Immunoglobulin G (IgG) |
Detection Range | 1.95-500 ng/mL |
Certified | ISO 9001:2008, ISO 13485:2003 |