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AVIVA Systems Biology
Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. The first acute phase protein to be recognized was discovered in humans by Tillet and Frances in 19301. Haptoglobin (Hp) is a heterogeneous plasma protein mostly synthesized by the liver. The haptoglobin monomer consist of two heavy chains, beta chains (40 kD) and two light chains, alpha chains, alpha 1 (9 kD) and alpha 2 (16 kD) that are linked disulfide bonds. The three major haptoglobin types are; Hp1-1 which is monomeric (98kD), Hp1-2 is polymeric at about 200 kD, and Hp2-2 at about 400 kD. The levels in serum rise quickly following acute tissue damage within 24 to 48 hours and also fall very rapidly once the stimulus is removed. In fact, Hp level are decreased in hemolytic anemia. Hp has a high affinity for hemoglobin (Hb) and its function appears to be to prevent loss of Hb in urine which would lead to loss of iron. Investigations over the past few years have shown that quantification of Hp in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in humans, but in companion animals and farm herds as well2.Immunoperoxidase Assay for Determination of Haptoglobin in Cat Samples
Cat
Cat
Haptoglobin (Cat) description of target: Cat Haptoglobingen symbol: HPGene ID: 100144610 ID:E3UTY9
Haptoglobin, HP, Zonulin
4.261 ng/ml
In Solution
96 Wells
1. DILUENTThe 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.2. WASH SOLUTIONThe 20X Wash Solution Concentrate is stable until the expiraᆲtion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.3. ENZYME-ANTIBODY CONJUGATEUndiluted horseradish peroxidase anti-Hp conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.4. CHROMOGEN-SUBSTRATE SOLUTIONThe Substrate Solution should be stored at 4-8C and is stable until the expiration date.5. STOP SOLUTIONThe Stop Solution should be stored at 4-8C and is stable until the expiration date.6. ANTI-CAT HAPTOGLOBIN ELISA MICRO PLATEAnti-Cat Hp coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.7. CAT HAPTOGLOBIN STANDARDSLong Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Pack size | 96 Wells |
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Brand | AVIVA Systems Biology |
Country of Manufacture | USA |
Sensitivity | 4.261 ng/ml |
Specific Against | Feline |
Antigen Name | Haptoglobin (Cat) |