Bovine Oxytocin EasyTestTM competitive ELISA kit

Brand

BioAim Scientific (Kanada)

short description

The BioAim Bovine Oxytocin EasyTestTM Competitive ELISA kit can quantitatively measure Oxytocin in Bovine serum or plasma. Other matrices, such as urine and tissue, may be suitable but have not been validated. It is a simple and rapid technology for the quantitation of antigen in a range of sample matrices. The whole process takes 2.5 hours with high accuracy and precision.
Research Area Peptide hormone;

specific against

Bovine

detection target

oxytocin(Oxytocin)
Background: Oxytocin (OXT) is a mammalian neurohypophysial hormone. Produced by the hypothalamus and stored and secreted by the posterior pituitary gland, oxytocin acts primarily as a neuromodulator in the brain. Oxytocin is a peptide of nine amino acids (a nonapeptide) with the sequence of cys –tyr – Ile – gln – asn – cys – pro – leu – gly. The structure of oxytocin is very similar to that of vasopressin, whose sequence differs from oxytocin by two amino acids. The oxytocin peptide is synthesized as an inactive precursor protein from the OXT gene. Its actions are mediated by a G-protein coupled receptor that requires Mg2+ and cholesterol. It belongs to the rhodopsin-type (class I) group of G-protein-coupled receptors. Oxytocin plays an important role in the neuroanatomy of intimacy, specifically in sexual reproduction of both sexes, in particular during and after childbirth; It is released in large amounts after distension of the cervix and uterus during labor, facilitating birth, maternal bonding, and, after stimulation of the nipples, lactation. Injected oxytocin analogues are used for labor induction and to support labor in case of difficult parturition. It has largely replaced ergometrine as the principal agent to increase uterine tone in acute postpartum hemorrhage. Recent studies have begun to investigate oxytocin’s role in various behaviors, including orgasm, social recognition, pair bonding, anxiety, and maternal behaviors.

alias

OT-NPI, OTNPI

sensitivity

80 pg/ml

detection range

0.024-100 ng/ml

sample type

Serum, plasma

pack size

1x96 Wells

Assay time

2.5 h

Principle of the Elisa

EIA

SwissProtID

P01175