17 Porcine OH Progesterone

Brand

GENWAY

Overview

Intended Use: Competitive immunoenzymatic colorimetric method for quantitative determination of 17 α OH Progesterone in serum or plasma. Introduction: 17-Hydroxyprogesterone (17-OH progesterone or 17OHP) is a C-21 steroid hormone produced in the adrenal gland and gonads during the synthesis of glucocorticoids and sex steroids. It is derived from progesterone via 17-hydroxylase a P450c17 enzyme or from 17-hydroxypregnenolone via 3β-hydroxysteroid dehydrogenase/delta 5-4 isomerase. 17α -OHP has no defined physiologic role except as a precursor molecule. Serum 17α -OHP levels are age-dependent with peak levels observed during fetal life and the immediate postnatal period. During the first week of life serum 17α -OHP levels fall ~50-fold as compared to cord blood values. A small transient increase occurs in male infants 30-60 days postnatally. Levels for both sexes remain at constant low levels during childhood and then progressively increase during puberty reaching adult levels of ~100 ng/dL (~3. 03 nmol/l). As with cortisol serum 17α -OHP levels normally have an ACTHdependent diurnal variation with peak levels in the morning and a nadir at night. In addition ovarian production of 17α -OHP increases during the luteal phase of the menstrual cycle. 17-hydroxyprogesterone is a natural progestin and in pregnancy increases in the third trimester primarily due to fetal adrenal production. Normal levels are 3-90 ng/dl in children and in women 15-70 ng/dl prior to ovulation and 35-290 ng/dl during the luteal phase. Measurements of levels of 17-hydroxyprogesterone are useful in the evaluation of patients with suspected congenital adrenal hyperplasia as the typical enzymes that are defective namely 21-hydroxylase and 11b-hydroxilase lead to a build-up of 17OHP. In contrast the rare patient with 17α-hydroxylase deficiency will have very low or undetectable levels of 17OHP. Elevated serum 17 α -OHP levels at baseline and/or after ACTH stimulation have also been reported in other forms of adrenal hyperplasia. Principle of the assay: Microtiter strip wells are precoated with anti-17 α OH Progesterone antibodies (solid-phase). 17 α OH Progesterone in the sample competes with added horseradish peroxidase labelled 17 α OH Progesterone (enzyme-labelled antigen) for antibody binding. After incubation a bound/free separation is performed by solid-phase washing. The immune complex formed by enzyme-labelled antigen is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is inversely proportional to the amount of 17 α OH Progesterone in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorption at 450 nm is read using an ELISA microwell plate reader. Storage and Stability: The reagents are stable up to the expiry date stated on the label when stored at 2-8 °C in the dark. Limitations of the Test: Bacterial contamination or repeated freeze-thaw cycles of the specimen may affect the absorbance values. References: Wisdom G. B. (1976) Clin. Chem. 22 (8) 1243 - 1255. De Villa G. O. et al. (1972) J. Clin. Endoc. Metab. 35 458. Hubl W. et al. (1982) Endokrinologie 79 (2) 165. Arakawa H. Maeda M. and Tsuji A. (1982) Chem. Pharm. Bull. Tokyo 30 (8) 3036. Riad-Fanny D. Read G. F. Walker R. F. and Griffiths K. (1982) Endocr. Reviews 3 (4) 304 - 367.

spezie

other

detection target

17 Porcine OH Progesterone

pack size

1x96 assay