Human Adiponectin PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human Adiponectin in cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates and urine.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Human Adiponectin
Protein name: Adiponectin
Gene Full Name: adiponectin, C1Q and collagen domain containing
abbreviation: ADIPOQ
Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226(19-244) amino acids with the molecular mass of 36KDa after glycosylation.
ID:Q15848
ID:9370

alias

30 kDa adipocyte complement related protein| 30 kDa adipocyte complement-related protein| ACDC| ACRP30| ADIPO_HUMAN| Adipocyte| Adipocyte C1q and collagen domain containing protein| Adipocyte complement related 30 kDa protein| Adipocyte complement related protein of 30 kDa| Adipocyte complement-related 30 kDa protein| Adiponectin| Adipoq| Adipose most abundant gene transcript 1| Adipose most abundant gene transcript 1 protein| Adipose specific collagen like factor| ADIPQTL1| ADPN| apM-1| APM1| C1q and collagen domain-containing protein| GBP28| Gelatin binding protein| Gelatin binding protein 28| Gelatin-binding protein

Origin

Nativ and recombinant human total Adiponectin

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, E19-N244

sensitivity

<60pg/ml

detection range

1.56ng/ml-100ng/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|13.8|37.2|67.3|13.1|36.8|65.7
Standard deviation|0.39|1.34|3.03|0.77|2.50|4.66
CV(%)|2.8|3.6|4.5|5.9|6.8|7.1

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO, E19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Adiponectin amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 100ng/ml, 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml human Adiponectin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human Adiponectin standard solution and each sample be measured in duplicate.

tipical result

Concentration(ng/ml)|0|1.56|3.12|6.25|12.5|25|50|100 O.D.|0.003|0.074|0.149|0.369|0.509|0.862|1.510|2.059

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human Adiponectin antibody|1
ST0000-100|Lyophilized recombinant human Adiponectin standard|100ng/tubex2
AR1107|Biotinylated anti- human Adiponectin antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4