Human ADAMTS4 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human ADAMTS4 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human ADAMTS4
Protein name: A disintegrin and metalloproteinase with thrombospondin motifs 4
Gene Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 4
abbreviation: ADAMTS4
Background: ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
ID:O75173
ID:9507

alias

A disintegrin and metalloproteinase with thrombospondin motifs 4|A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4|A disintegrin like and metalloprotease with thrombospondin type 1 motif 4|ADAM metallopeptidase with thrombospondin type 1 motif 4|ADAM TS 4|ADAM TS4|ADAM-TS 4|ADAM-TS4|ADAMTS 2|ADAMTS 4|ADAMTS 4|ADAMTS-4|ADAMTS2|ADAMTS4|ADAMTS4 protein|ADMP 1|ADMP-1|ADMP1|Aggrecanase 1|Aggrecanase 1|Aggrecanase-1|Aggrecanase1|ATS4_HUMAN|KIAA0688|KIAA0688

Origin

Nativ and recombinant human ADAMTS4

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

CHO, F213-C685

sensitivity

<20pg/ml

detection range

625pg/ml-40000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|5.9|12|21|6.5|14|23
Standard deviation|0.248|0.612|1.2|0.377|0.882|1.66
CV(%)|4.2|5.1|5.7|5.8|6.3|7.2

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human ADAMTS4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS4 has been precoated onto 96-well plates. Standards(CHO, F213-C685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS4 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 40000pg/ml, 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml human ADAMTS4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human ADAMTS4 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|625|1250|2500|5000|10000|20000|40000 O.D.|0.025|0.106|0.182|0.301|0.535|1.005|1.655|2.265

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human ADAMTS4 antibody|1
ST0000-40|Lyophilized recombinant human ADAMTS4 standard|40ng/tube�2
AR1107|Biotinylated anti- human ADAMTS4 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4