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Boster
For quantitative detection of human ADAMTS13 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human ADAMTS13Protein name: A disintegrin and metalloproteinase with thrombospondin motifs 13Gene Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 13abbreviation: ADAMTS13Background: ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP). ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity. ID:Q76LX8 ID:11093
A disintegrin and metalloproteinase with thrombospondin motifs 13|A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13|A disintegrin like and metalloprotease with thrombospondin type 1 motif 13|A disintegrin-like and metalloprotease (Reprolysin type) with thrombospondin type 1 motif 13|ADAM metallopeptidase with thrombospondin type 1 motif 13|ADAM TS|ADAM-TS 13|ADAM-TS13|ADAMTS 13|ADAMTS-13|ADAMTS13|ADAMTS13 protein|ATS13_HUMAN|C9orf8|EC 3.4.24.-|TTP|Von Willebrand factor cleaving protease|Von Willebrand factor cleaving protease|von Willebrand factor-cleaving protease|vWF cleaving protease|vWF CP|vWF-cleaving protease|vWF-CP|vWFCP
Nativ and recombinant human ADAMTS13
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
CHO, Q34-W688
<20pg/ml
0.78ng/ml-50ng/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(ng/ml)|5.62|10.1|25.5|6.23|12.1|26.4 Standard deviation|0.259|0.444|1.07|0.467|0.835|1.95 CV(%)|4.6|4.4|4.2|7.5|6.9|7.4
25-30
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human ADAMTS13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS13 has been precoated onto 96-well plates. Standards(CHO, Q34-W688) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS13 amount of sample captured in plate.
Aliquot 0.1ml per well of the 50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml human ADAMTS13 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See モSample Dilution Guidelineヤ above for details. We recommend that each human ADAMTS13 standard solution and each sample is measured in duplicate.
Concentration(ng/ml)|0|0.78|1.56|3.12|6.25|12.5|25|50 O.D.|0.051|0.101|0.134\0.243|0.439|0.829|1.482|2.472
Catalog number|Description|Quantity-|96-well plate precoated with anti- human ADAMTS13 antibody|1ST0000-50|Lyophilized recombinant human ADAMTS13 standard|50ng/tube�2AR1107|Biotinylated anti- human ADAMTS13 antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Pack size | 1x96 Wells |
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Brand | Boster |
Country of Manufacture | USA |
Sensitivity | <20pg/ml |
Specific Against | Human |
Antigen Name | A disintegrin and metalloproteinase with thrombospondin motifs 13 (ADAMTS13) |
Detection Range | 0.78ng/ml-50ng/ml |
Alias | A disintegrin and metalloproteinase with thrombospondin motifs 13, A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13, A disintegrin like and metalloprotease with thrombospondin type 1 motif 13, A disintegrin-like |