Human ADAMTS1 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human ADAMTS1 in cell culture supernates, serum and plasma(heparin).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human ADAMTS1
Protein name: A disintegrin and metalloproteinase with thrombospondin motifs 1
Gene Full Name: ADAM metallopeptidase with thrombospondin type 1 motif, 1
abbreviation: ADAMTS1
Background: ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What′s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.
ID:Q9UHI8
ID:9510

alias

A disintegrin and metalloproteinase with thrombospondin motifs 1|A disintegrin like and metalloprotease with thrombospondin type 1 motif 1|A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1|ADAM metallopeptidase with thrombospondin type 1 motif 1|ADAM TS 1|ADAM TS1|ADAM-TS 1|ADAM-TS1|ADAMTS 1|ADAMTS|ADAMTS-1|Adamts1|ATS1_HUMAN|C3 C5|KIAA1346|METH 1|METH-1|METH1

Origin

Nativ and recombinant human ADAMTS1

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, F253-A734

sensitivity

<10pg/ml

detection range

93.8pg/ml-6000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1208|2741|4137|1850|3035|4726
Standard deviation|42.28|117.9|202.7|98.05|176.03|302.5
CV(%)|3.5|4.3|4.9|5.3|5.8|6.4

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human ADAMTS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS1 has been precoated onto 96-well plates. Standards(NSO, F253-A734) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS1 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.7pg/m human ADAMTS1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human ADAMTS1 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|93.7|187.5|375|750|1500|3000|6000 O.D.|0.026|0.096|0.160|0.278|0.513|0.920|1.599|2.476

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human ADAMTS1 antibody|1
ST0000-10|Lyophilized recombinant human ADAMTS1 standard|10ng/tube�2
AR1107|Biotinylated anti- human ADAMTS1 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4