Human ADAM9 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human ADAM9 in cell culture supernates, serum and plasma(heparin).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human ADAM9
Protein name: Disintegrin and metalloproteinase domain-containing protein 9
Gene Full Name: ADAM metallopeptidase domain 9
abbreviation: ADAM9
Background: Disintegrin and metalloproteinase domain-containing protein 9, also known as MCMP or MDC9 is an enzyme that in humans is encoded by the ADAM9 gene. This gene encodes a member of the ADAM('a disintegrin and metalloproteinase') family and exhibits a number of characteristics of class III reprolysins. This gene is mapped to 8p11.22. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis.
ID:Q13443
ID:8754

alias

ADAM 9|ADAM metallopeptidase domain 9|Adam9|ADAM9_HUMAN|Cellular disintegrin-related protein|Cone rod dystrophy 9|CORD9|Disintegrin and metalloproteinase domain-containing protein 9|MCMP|MDC9|Meltrin-gamma|Metalloprotease/disintegrin/cysteine-rich protein 9|Mltng|Myeloma cell metalloproteinase

Origin

Nativ and recombinant human ADAM9

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, A29-D697

sensitivity

<10pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|145|363|594|176|380|658
Standard deviation|7.54|20.69|36.23|11.44|28.12|51.98
CV(%)|5.2|5.7|6.1|6.5|7.4|7.9

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human ADAM9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAM9 has been precoated onto 96-well plates. Standards(NSO, A29-D697) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAM9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAM9 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human ADAM9 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human ADAM9 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.042|0.092|0.121|0.194|0.319|0.570|1.023|2.010

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human ADAM9 antibody|1
ST0000-10|Lyophilized recombinant human ADAM9 standard|10ng/tube�2
AR1107|Biotinylated anti- human ADAM9 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4