CytoSelect™ BrdU Cell Proliferation ELISA Kit

Brand

Cell Biolabs

short description

Detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody
The CytoSelect™ BrdU Cell Proliferation ELISA Kit detects BrdU incorporated into cellular DNA during cell proliferation using an anti-BrdU antibody.  When cells are incubated in media containing BrdU, the pyrimidine analog is incorporated in place of thymidine into the newly synthesized DNA of proliferating cells.  Once the labeling media is removed, the cells are fixed and the DNA is denatured in one step with a fix/denature solution (denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection).  Then an anti-BrdU mouse monoclonal antibody is added followed by an HRP conjugated secondary antibody to detect the incorporated BrdU.  The magnitude of the absorbance for the developed color is proportional to the quantity of BrdU incorporated into cells and can be directly correlated to cell proliferation.
Research area: Cell Based Assays

spezie

other

detection target

CytoSelect BrdU Cell Proliferation

sample type

Compatible with plasma, serum or cell culture supernatant samples 

pack size

96 assays

storage and Stability

Upon receipt, store the 1000X BrdU Solution and Anti-BrdU Monoclonal Antibody at -20°C. Store all other components at 4°C.

Shipping Temp

Room Temp

Kit Content

BrdU standard included