Rat Visfatin EasyTestTM competitive ELISA kit

Brand

BioAim Scientific (Kanada)

short description

The BioAim rat Visfatin EasyTestTM Competitive ELISA kit can quantitatively measure Visfatin in rat serum or plasma. Other matrices, such as urine and tissue, may be suitable but have not been validated. It is a simple and rapid technology for the quantitation of antigen in a range of sample matrices. The whole process takes 2 hours with high accuracy and precision.
Research Area Cancer Biomarker;

specific against

Rat

detection target

nicotinamide phosphoribosyltransferase(Visfatin)
Background: Visfatin (also known as PBEF and Namp) is an enzyme that in humans is encoded by the PBEF1 gene. This protein has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis. It is downregulated by an increase of miR-34a in obesity via a 3’UTR functional binding site of NAMPT mRNA resulting in a reduction of NAD (+) and decreased SIRT1 activity. Visfatin catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

alias

Pbef, Pbef1

sensitivity

130 pg/ml

detection range

9.8-40000 pg/ml

sample type

Serum, plasma

pack size

1x96 Wells

Assay time

2.5 h

Principle of the Elisa

EIA

SwissProtID

Q80Z29