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BioAim Scientific (Kanada)
The Bioaim Rat VEGF ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of VEGF in rat serum, plasma and cell culture media. An antibody specific for rat VEGF was coated on a 96-well plate. Standards and samples are added to the wells and any VEGF present binds to the immobilized antibody. The wells are washed and biotinylated anti-rat VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of VEGF present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area wound healing; tumor growth; vascular disease' Cancer biomarker;
Rat
vascular endothelial growth factor A [( VEGF-A ) Background: VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis. In addition to binding to VEGFRs, VEGF binds to receptor complexes consisting of both neuropilins and VEGFRs. This receptor complex has increased VEGF signalling activity in endothelial cells (blood vessels). Neuropilins (NRP) are pleitrophic receptors and therefore other molecules may interfere with the signalling of the NRP/VEGFR receptor complexes. For example, Class 3 semaphorins compete with VEGF165 for NRP binding and could therefore regulate VEGF-mediated angiogensis.
VEGF-A, VEGF164, VPF, Vegf
< 1 pg/ml
0.82-200 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P16612
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | < 1 pg/ml |
Specific Against | Rat |
Antigen Name | vascular endothelial growth factor A [ |
Detection Range | 0.82-200 pg/ml |
Alias | VEGF-A, VEGF164, VPF, Vegf |