BioAim Scientific (Kanada)
The Bioaim Rat MMP-8 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MMP-8 in rat serum, plasma and cell culture media. An antibody specific for rat MMP-8 was coated on a 96-well plate. Standards and samples are added to the wells and any MMP-8 present binds to the immobilized antibody. The wells are washed and biotinylated anti-rat MMP-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MMP-8 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; Cancer Biomarker;
matrix metallopeptidase 8 ( MMP-8 ) Background: Neutrophil collagenase, also known as matrix metalloproteinase-8 (MMP-8) or PMNL collagenase (MNL-CL), is a collagen cleaving enzyme which is present in the connective tissue of most mammals. Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-8 (neutrophil collagenase, collagenase 2) is expressed in neutrophils, where it is stored in specific granules. MMP-8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-alpha and GM-CSF. MMP-8 is capable of cleaving types I, II, and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, beta -casein, and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP-8 has a high capacity for infiltrating connective tissue and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis.
Serum, plasma, Cell culture supernatant
|Antigen Name||matrix metallopeptidase 8|
|Detection Range||0.05-40 ng/ml|