×

Registrierung

Profile Informationen

Login Daten

oder Einloggen

Vorname ist erforderlich!
Nachname ist erforderlich!
Vorname ist ungültig!
Nachname ist ungültig!
Dies ist keine E-Mail-Adresse!
E-Mail-Adresse ist erforderlich!
Dies E-Mail-Adresse ist bereit registriert!
Passwort ist erforderlich!
Geben Sie ein gültiges Passwort ein!
Bitte geben Sie 6 oder mehr Zeichen ein!
Bitte geben Sie 16 oder weniger Zeichen ein!
Passwörter sind nicht dasselbe!
Geschäftsbedingungen sind erforderlich!
E-Mail oder Passwort ist falsch!

Rat MCP-1 ELISA Kit

Product Code: BAS-3030103

Verfügbarkeit: Auf Lager

Rat MCP-1 ELISA Kit

Bewerten Sie dieses Produkt als Erster

OR

Fragen zur Lieferzeit

Angebot anfordern

Beschreibung

Details

Rat MCP-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Rat MCP-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of MCP-1 in rat serum, plasma and cell culture media. An antibody specific for rat MCP-1 was coated on a 96-well plate. Standards and samples are added to the wells and any MCP-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-rat MCP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of MCP-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Inflammation;

specific against

Rat

detection target

C-C motif chemokine ligand 2 ( MCP-1 )
Background: CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding. CCL2 is implicated in pathogeneses of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis. Administration of anti-CCL2 antibodies in a model of glomerulonephritis reduces infiltration of macrophages and T cells, reduces crescent formation, as well as scarring and renal impairment.

alias

MCP-1, Scya2, Sigje

sensitivity

15 pg/ml

detection range

24.7-18000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P14844

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 15 pg/ml
Specific Against Rat
Antigen Name C-C motif chemokine ligand 2 
Detection Range 24.7-18000 pg/ml
Alias MCP-1, Scya2, Sigje
Bewertungen
Tags

Produkttags

Verwenden Sie Leerzeichen um Tags zu trennen. Verwenden Sie Apostrophe (') für Phrasen.

Datasheet

Video