BioAim Scientific (Kanada)
The Bioaim Rat L-Selectin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of L-Selectin in rat serum, plasma and cell culture media. An antibody specific for rat L-Selectin was coated on a 96-well plate. Standards and samples are added to the wells and any L-Selectin present binds to the immobilized antibody. The wells are washed and biotinylated anti-rat L-Selectin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of L-Selectin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; Cell adhesion;
selectin L(L-selectin) Background: The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells.
A.11, L-selectin, LECAM-1
Serum, plasma, Cell culture supernatant
|Antigen Name||selectin L|
|Detection Range||8.2-6000 pg/ml|
|Alias||A.11, L-selectin, LECAM-1|