Rat IL-3 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat IL-3 in cell culture supernates and serum.

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

rat IL-3
Protein name: Interleukin-3
Gene Full Name: interleukin 3
abbreviation: IL3
Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa.
ID:P04823
ID:24495

alias

Burst promoting activity| Colony stimulating factor multiple| Eosinophil colony stimulating factor| Hematopoietic growth factor| IL 3| IL-3| IL3| IL3_HUMAN| Interleukin 3| Interleukin-3| Mast cell growth factor| MCGF| Multi CSF| Multilineage colony stimulating factor| Multipotential colony stimulating factor| Multipotential colony-stimulating factor| P cell stimulating factor| P-cell-stimulating factor| Thy1 inducing factor

Origin

Nativ and recombinant rat IL-3

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, I27-C169

sensitivity

<10pg/ml

detection range

7.8pg/ml-500pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|44|166|347|65|191|386
Standard deviation|2.29|11.8|23.25|3.77|15.1|27.02
CV(%)|5.2|7.1|6.7|5.8|7.9|7

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat IL-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-3 has been precoated onto 96-well plates. Standards(E.coli, I27-C169) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-3 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml, 7.8pg/ml rat IL-3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates or serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each rat IL-3 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|7.8|15.6|31.3|62.5|125|250|500 O.D.|0.011|0.059|0.096|0.184|0.299|0.767|1.478|2.267

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat IL-3 antibody|1
ST0000-10|Lyophilized recombinant rat IL-3 standard|10ng/tubex2
AR1107|Biotinylated anti- rat IL-3 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4