Rat Clusterin ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Rat Clusterin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Clusterin in rat serum, plasma and cell culture media. An antibody specific for rat Clusterin was coated on a 96-well plate. Standards and samples are added to the wells and any Clusterin present binds to the immobilized antibody. The wells are washed and biotinylated anti-rat Clusterin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Clusterin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Apoptosis; Cancer Biomarker; Lipid metabolism;

specific against

Rat

detection target

Clusterin(Clusterin)
Background: Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40,40, is a secreted multifunctional protein that binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. CLU is a molecular chaperone responsible for aiding protein folding of secreted proteins, and its three isoforms have been differentially implicated in pro- or antiapoptotic processes. Through this function, CLU is involved in many diseases related to oxidative stress, including neurodegenerative diseases, cancers, inflammatory diseases, and aging. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis. Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development. It is secreted as a disulfide-linked heterodimer of alpha and beta chains and circulates as a component of high density lipoprotein (HDL) particles.

alias

APOJ, CLI, DAG, RATTRPM2B, SGP-2, SGP2, SP-40, SP40, TRPM-2, TRPM2B, Trpm2, Trpmb

sensitivity

0.8 ng/ml

detection range

1.31-320 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P05371