Rat CD36/SR-B3 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of rat CD36 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Rat

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

rat CD36
Protein name: Platelet glycoprotein 4
Gene Full Name: CD36 molecule (thrombospondin receptor)
abbreviation: CD36
Background: CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.
ID:Q07969
ID:29184

alias

CD36 antigen|CD36 antigen (collagen type I receptor thrombospondin receptor)|CD36 molecule|CD36 molecule (thrombospondin receptor)|CHDS7|Cluster determinant 36|FAT|Fatty acid translocase|Glycoprotein IIIb|GP3B|GP4|GPIIIB|GPIV|Leukocyte differentiation antigen CD36|PAS 4 protein|PAS IV|PASIV|Platelet collagen receptor|Platelet glycoprotein 4|Platelet glycoprotein IV|SCARB3|Scavenger receptor class B member 3|Thrombospondin receptor

Origin

Nativ and recombinant rat CD36

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

CHO, G30-K439

sensitivity

<10pg/ml

detection range

156pg/ml-10000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|243|936|3165|195|1201|4201
Standard deviation|8.01|36.50|136.09|11.89|62.45|239.45
CV(%)|3.3|3.9|4.3|6.1|5.2|5.7

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s rat CD36 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD36 has been precoated onto 96-well plates. Standards(CHO, G30-K439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD36 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD36 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat CD36 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each rat CD36 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000 O.D.|0.072|0.182|0.289|0.435|0.689|1.087|1.539|2.137

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- rat CD36 antibody|1
ST0000-10|Lyophilized recombinant rat CD36 standard|10ng/tube�2
AR1107|Biotinylated anti- rat CD36 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4