Porcine IP-10 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Porcine IP-10 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IP-10 in porcine serum, plasma and cell culture media. An antibody specific for porcine IP-10 was coated on a 96-well plate. Standards and samples are added to the wells and any IP-10 present binds to the immobilized antibody. The wells are washed and biotinylated anti-porcine IP-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IP-10 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Inflammation;

specific against

Porcine

detection target

C-X-C motif chemokine ligand 10 (IP-10)
Background: IP-10 was originally identified as an IFN-gamma-inducible gene in monocytes, fibroblasts and endothelial cells. The mouse homolog of human IP-10, CRG-2, shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of IP-10 identified the protein as a member of the CXC chemokine subfamily. CXCL10 is secreted by several cell types in response to IFN-β. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. This chemokine elicits its effects by binding to the cell surface chemokine receptor CXCR3.

sensitivity

0.4 ng/ml

detection range

0.41-100 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

K7GRV1