Porcine CCL4 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Porcine CCL4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of CCL4 in porcine serum, plasma and cell culture media. An antibody specific for porcine CCL4 was coated on a 96-well plate. Standards and samples are added to the wells and any CCL4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-porcine CCL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of CCL4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Inflammation;

specific against

Porcine

detection target

chemokine (C-C motif) ligand 4 (CCL4)
Background: CCL4/MIP-1 beta is a beta chemokine that is secreted at sites of inflammation by activated leukocytes, lymphocytes, vascular endothelial cells, and pulmonary smooth muscle cells. It attracts a variety of immune cells to sites of microbial infection as well as to other pathologic inflammation such as allergic asthma and ischemic myocardium. CCL4 is secreted from activated monocytes as a heterodimer with CCL3/MIP-1 alpha. It signals through CCR5, and an N-terminally trimmed form additionally interacts with CCR1 and CCR2. In humans, the ability of CCL4 to bind CCR5 inhibits the cellular entry of M-tropic HIV-1 which utilizes CCR5 as a coreceptor. CCL4 has been shown to interact with CCL3.

alias

MIP-1BETA, MIP1b, SCY4A

sensitivity

0.2 ng/ml

detection range

0.205-50 ng/ml

certificate

ISO9001:2008

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q711P4