The TBARS (Thiobarbituric Acid Reactive Substances) assay is well-established for screening and monitoring lipid peroxidation. MDA forms a 1:2 adduct with thiobarbituric acid, the MDA-TBA adduct can then be measured. Our OxiSelect TBARS Assay Kit provides a much more user-friendly protocol to measure the MDA-TBA adduct. Reaction volumes are much smaller than the traditional assay, so much less sample is required. Also, reactions can be performed in standard polypropylene tubes - no glass tubes or glass marbles are required. important Note: MDA adducts are not stable long term. For best results test all samples immediately upon collection, or freeze them at -80C for up to one month. MDA may be degraded in samples that have been frozen for longer periods, in such cases more reliable results may be obtained from more stable markers of oxidative stress such as protein carbonyl, 8-OHdG or 4-HNE.
1. Quick 30 minute assessment for oxidative stress
2. User friendly protocol uses smaller reaction volumes in a 96-well format
3. Does not require glass tubes or marbles