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BioAim Scientific (Kanada)
The Bioaim Mouse TRANCE ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TRANCE in mouse serum, plasma and cell culture media. An antibody specific for mouse TRANCE was coated on a 96-well plate. Standards and samples are added to the wells and any TRANCE present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TRANCE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TRANCE present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Hematologic Cancer; Osteoclast differentiation;
Mouse
tumor necrosis factor (ligand) superfamily, member 11( TRANCE ) Background: TRANCE (TNF-related activation-induced cytokines), also known as RANK Ligand, OPGL, or ODF, is a TNF superfamily ligand produced by osteoblasts, bone marrow stromal cells, and activated T cells. It signals through RANK and is antagonized by the decoy receptor Osteoprotegerin. TRANCE stimulates bone resorption, is required for osteoclast differentiation, and is associated with the development of osteoporosis, vascular calcification, osteopetrosis, and progestin-induced breast cancer. It also mediates body temperature regulation and is essential for the development of a lactating mammary gland.
Ly109l, ODF, OPG, OPGL, RANKL, Trance
1 pg/ml
2.7-2000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
O35235
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 1 pg/ml |
Specific Against | Mouse |
Antigen Name | tumor necrosis factor (ligand) superfamily, member 11 |
Detection Range | 2.7-2000 pg/ml |
Alias | Ly109l, ODF, OPG, OPGL, RANKL, Trance |