BioAim Scientific (Kanada)
The Bioaim Mouse TNFSF4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TNFSF4 in mouse serum, plasma and cell culture media. An antibody specific for mouse TNFSF4 was coated on a 96-well plate. Standards and samples are added to the wells and any TNFSF4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TNFSF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TNFSF4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Apoptosis;
tumor necrosis factor (ligand) superfamily, member 4 (TNFSF4 ) Background: OX40 Ligand (OX40L), also known as gp34, is a member of the TNF superfamily. Human OX40L shares 46% amino acid sequence identity with its mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similar to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily, which is expressed predominantly on activated CD4+ T cells. Various single nucleotide polymorphisms (SNPs) of the OX40L gene have been identified. For some of them association with systemic lupus erythematosus has been reported.
Ath-1, Ath1, CD134L, OX-40L, Ox40l, TXGP1, Tnlg2b, Txgp1l, gp34
Serum, plasma, Cell culture supernatant
|Antigen Name||tumor necrosis factor (ligand) superfamily, member 4|
|Detection Range||32.8-8000 pg/ml|
|Alias||Ath-1, Ath1, CD134L, OX-40L, Ox40l, TXGP1, Tnlg2b, Txgp1l, gp34|