Mouse TNFRSF7/CD27 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of mouse TNFRSF7 in cell culture supernates and serum.

spezie

Mouse

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

Mouse TNFRSF7
Protein name: CD27 antigen
Gene Full Name: CD27 molecule
abbreviation: CD27
Background: CD27 antigen, also called CD27 or TNFRSF7 is a tumor necrosis factor receptor. This gene is mapped to 12p13.31. The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein(SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.
ID:P41272
ID:21940

alias

CD 27|Cd27|CD27 antigen|CD27 molecule|CD27_HUMAN|CD27L receptor|LPFS2|MGC20393|OTTHUMP00000238557|S152|T cell activation antigen CD27|T cell antivation antigen S152|T-cell activation antigen CD27|T14|TNFRSF 7|TNFRSF7|TNFSF7|Tp 55|Tp55|Tumor necrosis factor receptor superfamily member 7

Origin

Nativ and recombinant mouse TNFRSF7

Antibodies

a monoclonal antibody from rat|a biotinylated polyclonal antibody from goat

Immunogene

NSO, T21-R182

sensitivity

<10pg/ml

detection range

62.5pg/ml-4000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|533|1626|2563|658|1856|2731
Standard deviation|22.4|56.91|138.4|39.5|89.1|180.3
CV(%)|4.2|3.5|5.4|6|4.8|6.6

TMB(tetramethylbenzidine) incubation time

15-20

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s mouse TNFRSF7 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF7 has been precoated onto 96-well plates. Standards(NSO, T21-R182) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF7 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse TNFRSF7 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates and serum to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each mouse TNFRSF7 standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.082|0.125|0.166|0.223|0.412|0.739|1.21|2.152

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- mouse TNFRSF7 antibody|1
ST0000-10|Lyophilized recombinant mouse TNFRSF7 standard|10ng/tubex2
AR1107|Biotinylated anti- mouse TNFRSF7 antibody|130ul(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4