Mouse TNF-alpha EasyTestTM ELISA kit

Brand

BioAim Scientific (Kanada)

short description

The BioAim Mouse TNF-alpha EasyTestTM ELISA kit can quantitatively measure TNF-alpha in Mouse serum or plasma. It is a simple and rapid technology for the quantitation of antigen in a range of sample matrices. The whole process takes less than 1.5 hours with high accuracy and precision. EasyTestTM ELISA is faster and easier to perform than standard format ELISA with less reagent handling and fewer pipetting steps.
Research Area Apoptosis; Cancer Biomarker; Cell adhesion; Inflammation;

specific against

Mouse

detection target

tumor necrosis factor(TNF-α)
Background: Tumor necrosis factor alpha (TNF-α), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-α is produced by a wide variety of immune and epithelial cell types. Human TNF-α shares 97% aa sequence identity with rhesus and 71% – 92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-α. TNF-α trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers. TNF-α regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNF-α is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism.

alias

DIF, TNF-a, TNF-alpha, TNFSF2, TNFalphaa, Tnfsf1a, Tnlg1f, Tnf

sensitivity

20 pg/ml

detection range

49.2-12000 pg/ml

sample type

Serum

pack size

1x96 Wells

Assay time

1.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P06804