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Mouse TIMP-2 ELISA Kit

Product Code: BAS-3020013

Verfügbarkeit: Auf Lager

Mouse TIMP-2 ELISA Kit

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Beschreibung

Details

Mouse TIMP-2 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Mouse TIMP-2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TIMP-2 in mouse serum, plasma and cell culture media. An antibody specific for mouse TIMP-2 was coated on a 96-well plate. Standards and samples are added to the wells and any TIMP-2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TIMP-2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Cancer Biomarker;

specific against

Mouse

detection target

tissue inhibitor of metalloproteinase 2 ( TIMP-2 )
Background: The Bioaim Mouse TIMP-2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TIMP-2 in mouse serum, plasma and cell culture media. An antibody specific for mouse TIMP-2 was coated on a 96-well plate. Standards and samples are added to the wells and any TIMP-2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TIMP-2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.

alias

D11Bwg1104e,Timp-2

sensitivity

40 pg/ml

detection range

0.082-60 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P25785

Weitere Informationen

Weitere Informationen

Menge 1x96 Wells
Hersteller BioAim Scientific
Herstellerland Kanada
Sensitivity 40 pg/ml
Specific Against Mouse
Antigen Name tissue inhibitor of metalloproteinase 2 
Detection Range 0.082-60 ng/ml
Alias D11Bwg1104e,Timp-2
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