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BioAim Scientific (Kanada)
The Bioaim Mouse TIMP-2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TIMP-2 in mouse serum, plasma and cell culture media. An antibody specific for mouse TIMP-2 was coated on a 96-well plate. Standards and samples are added to the wells and any TIMP-2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TIMP-2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; Cancer Biomarker;
Mouse
tissue inhibitor of metalloproteinase 2 ( TIMP-2 ) Background: The Bioaim Mouse TIMP-2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of TIMP-2 in mouse serum, plasma and cell culture media. An antibody specific for mouse TIMP-2 was coated on a 96-well plate. Standards and samples are added to the wells and any TIMP-2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of TIMP-2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
D11Bwg1104e,Timp-2
40 pg/ml
0.082-60 ng/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P25785
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 40 pg/ml |
Specific Against | Mouse |
Antigen Name | tissue inhibitor of metalloproteinase 2 |
Detection Range | 0.082-60 ng/ml |
Alias | D11Bwg1104e,Timp-2 |